Coding

Part:BBa_K2689000:Design

Designed by: Manuel Lubetzki   Group: iGEM18_Tuebingen   (2018-10-09)


pHluorin2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No additional mutations were introduced. The sequence starts with a short GS linker so it can be assembled via Gibson-Assembly. Additionally a His-Tag was introduced to facilitate purification.


Source

While GFP stems from Aequorea victoria, pHluorin and pHluorin2 were rationally designed from GFP. We copied it from the original publication by Matthew Mahon. https://dx.doi.org/10.4236%2Fabb.2011.23021

References

Matthew J. Mahon (2011) pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein - https://dx.doi.org/10.4236%2Fabb.2011.23021